Mr. Eldar Garayev
Azerbaijan Medical University, Azerbaijan
Abstract Title: “L-FBAC- Limonium Caspium Flavonoids as a Rich Source of Biologically Active Compounds
Biography: Eldar Garayev (1964) Professor, Head of Pharmaceutical Toxicology and Chemistry Department, Azerbaijan Medical University, Azerbaijan. Professor Eldar Garayev has more than 200 scientific works, including: 6 textbooks, 8 patents (joint). Germany, Turkey, Greece, Russia, Ukraine, Belorussia, Uzbekistan, Kazakhstan, Georgia and others in impact factor journals. Social activities: Chairman of the Health Ethics Committee of Health Ministry, Azerbaijan Repub.; Member of the Scientific Council of Azerbaijan Medical University; Member of the Scientific Council of AMU; Scientific editor of “Journal of Azerbaijan Medical University”, Member of Editorial Board of "Azerbaijan Pharmaceutical and Pharmacoterapy Journal", Member of Editorial Board of "Phytoterapia Chasopis” (Ukraine) Journal, Full member of SOT (Society of Toxicology)
Research Interest: Flavonoids are currently of great interest and have broad research prospects, as these compounds possess significant potential in the fields of medicine and pharmacy. In this regard, we have focused on this direction and set the objective of isolating and studying biological activity of flavonoids from Limonium caspium (Willd.) P.Fourn., from the rich flora of Azerbaijan. The antioxidant properties of the flavonoids myricetin, myricitrin, and myricetin-3'-O-sulfate, obtained from the roots of L. caspium were comparatively studied in vitro. DPPH analysis was performed according to the analysis of Blios for the determination of antioxidant activity of flavonoids. The absorbance of the samples was measured at a wavelength of 515 nm. The antioxidant activity and IC50 (the relationship between the concentration of the sample for 50% inhibition of radicals) were calculated using the established formulas. Based on the analysis, the IC₅₀ values characterizing the level of antioxidant activity were calculated as follows: myricetin – 7.81 µg/mL, myricitrin – 6.34 µg/mL, and myricetin-3'-O-sulfate – 6.2 µg/mL. Gallic acid was used as a control sample and showed an IC₅₀ value of 3.48 µg/mL. The obtained results demonstrated that all three compounds possess sufficient level of antioxidant activity. Among the predicted pharmacological effects obtained by PASS Online program for the compounds the hemostatic activity is of particular interest. To confirm this effect via in vivo studies, myricetin-3'-O-sulfate compound was administered to laboratory animals (Rattus norvegicus). Hemostatic indicators (coagulation parameters) were evaluated in all rats. A significant increase in platelet count (PLT) was observed among the coagulation parameters. This confirms that the in silico predicted hemostatic effect was also validated by in vivo experiments.